Characterizing tumor-infiltrating lymphocyte (TIL) activity is essential for understanding anti-tumor immune responses and optimizing immuno-oncology therapeutics. AutoTIL™ Microtumors co-cultured with autologous TILs recapitulate the tumor-immune microenvironment, preserving critical cross-talk interactions and enabling quantitative evaluation of TIL infiltration, activation, and cytotoxicity.
Real-time live-cell imaging, combined with confocal microscopy, provides high-resolution measurement of TIL-mediated tumor cell killing, supporting precise assessment of therapeutic efficacy and dose-response relationships. Integration with proteomic profiling of signaling pathways and protein expression allows mechanistic interpretation of TIL activity and identification of pathways that can be targeted to enhance immune responses.
Functional co-culture assays under treatment conditions enable systematic evaluation of therapeutic candidates for their capacity to enhance TIL-mediated cytotoxicity or overcome tumor immune evasion. These approaches generate reproducible, actionable datasets that facilitate early stratification of responders and non-responders, guide candidate prioritization, and support mechanism-driven development of combination immunotherapies.
Main Benefit
Provide actionable, mechanistic insights into TIL function that guide candidate prioritization and accelerate immuno-oncology development.
Schedule an individual life event with a scientist at Assay Engineers to discuss benefits of AutoTIL™ Microtumors with autologous TILs.
